FITC Antikörper (Alkaline Phosphatase (AP))

Produktnummer ABIN401116

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch FITC in WB, IHC (fro), EIA und ID. Er zeigt eine Reaktivität gegenüber .

Kurzübersicht für FITC Antikörper (Alkaline Phosphatase (AP)) (ABIN401116)

Target: FITC (Fluorescein Isothiocyanate (FITC))

Reaktivität: Bitte anfragen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser FITC Antikörper ist konjugiert mit Alkaline Phosphatase (AP)

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunodiffusion (ID)

Produktdetails anti-FITC Antikörper (Alkaline Phosphatase (AP))

Aufreinigung: Affinity Chromatography

Immunogen: Fluorescein conjugated to Goat IgG

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/2,000 - 1/10,000. Western Blot: 1/500 - 1/2,500. Immunohistochemistry: 1/200 - 1/: 1,000. Immunodiffusion.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.005 M Magnesium Chloride, 0.0005 M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0 containing 10 mg/mL BSA (IgG and Protease Free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu FITC

Target: FITC (Fluorescein Isothiocyanate (FITC))

Andere Bezeichnung: FITC

Substanzklasse: Chemical

Hintergrund: Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is? pH sensitive and is significantly reduced below? pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.Synonyms: Fluorescein Isothiocyanate